Confocal and Multiphoton Microscopy
Services
The confocal and multiphoton microscopy laboratory supports campuswide research programs funded by the National Institutes of Health (NIH) and other organizations, including the UMKC Center of Excellence in the Study of Dental and Musculoskeletal Tissues (CEMT). Its services are also available to the academic and commercial communities in the Kansas City area.
Our services include a wide range of traditional high-resolution microscopy and spectral imaging projects and supports biophysical methods and applications development. Image analysis automation and high content microscopy image analysis services are also available.
Microscopes and Imaging
Leica inverted SP8 Stellaris confocal microscope
- Live cell imaging (incubation chamber with co2, temperature and humidity control)
- Multispectral confocal imaging of fixed cells and tissue samples
- Tiled image capture for high-resolution imaging of large areas
- Optical sectioning and 3D image capture (also in timelapse)
- Spectral profiling
- Förster resonance energy transfer
- Fluorescence recovery after photobleaching
- Fluorescence loss in photobleaching
- Fluorescence lifetimes imaging microscopy
- High-speed imaging for live cell kinetics
- Multipoint timelapse movie collection.
- Physiological indicators imaging (for example, calcium imaging and ph imaging)
- Realtime optical deconvolution ( >120 nm full width at half maximum)
Leica upright SP8 multiphoton and confocal microscope
- Multiphoton imaging and second harmonic generation imaging.
- Intravital imaging
- Live cells and fixed tissue imaging
- Förster resonance energy transfer (fret)
- Fluorescence recovery after photobleaching (frap)
- Fluorescence loss in photobleaching (flip)
- High speed imaging for live cell kinetics
- Multi-point time-lapse movie collection.
- Physiological indicators imaging (for example, calcium imaging and ph imaging)
Keyence BZ-X810 epifluorescence high content imaging system
- Brightfield imaging with color camera for tissue histology
- Widefield multi-color fluorescence imaging
- Image tiling
- High-content imaging cytometry analysis
Registration and Reservations
We offer assisted operation, independent operation and advanced user operation. You must first register before scheduling a reservation. Training is performed by one of the co-directors, so you must reserve time with both the instrument and your trainer.
Hourly Fees
Leica SP8 Stellaris Confocal Microscope
| User category | UMKC | Non-UMKC Academic | Commercial |
|---|---|---|---|
| Assisted | $40.00 | $50.00 | $120.00 |
| Independent | $25.00 | $35.00 | $85.00 |
| Time series and off-peak hours | $15.00 | $20.00 | N/A |
| Fee for service | Contact us | $95.00 | $170.00 |
Time series extends more than eight hours ($500 cap for long-term, three- to four-day timelapse experiments). Off-peak hours begin after 4 p.m. and end at 10 a.m. Only advanced users with access to the building may use instruments during off-peak hours.
Leica SP8 Multiphoton Microscope
| User category | UMKC | Non-UMKC Academic | Commercial |
|---|---|---|---|
| Assisted | $55.00 | $65.00 | $180.00 |
| Independent | $35.00 | $45.00 | $110.00 |
| Time series and off-peak hours | $25.00 | $30.00 | N/A |
| Fee for service | Contact us | $120.00 | $220.00 |
Time series extends more than eight hours ($600 cap for long-term, three- to four-day timelapse experiments). Off-peak hours begin after 4 p.m. and end at 10 a.m. Only advanced users with access to the building may use instruments during off-peak hours.
Keyence BZ-X810 High Content Imaging System
| User category | UMKC | Non-UMKC Academic | Commercial |
|---|---|---|---|
| Assisted | $15.00 | $20.00 | Contact us |
| Independent | $10.00 | $15.00 | Contact us |
| Time series and off-peak hours | $5.00 | N/A | Contact us |
| Fee for service | Contact us | $75.00 | Contact us |
Time series extends more than eight hours ($600 cap for long-term, three- to four-day timelapse experiments). Off-peak hours begin after 4 p.m. and end at 10 a.m. Only advanced users with access to the building may use instruments during off-peak hours.
New investigators applying for NIH funding may contact us for a fee waiver for an initial period of use not to exceed 20 hours in order to generate preliminary data for grant applications.
Safety and Approvals
As part of the user registration process, biosafety concerns will be reviewed with the PI and appropriate IRB, biosafety or IACUC approval will be confirmed. Before any biohazardous samples are viewed on the confocal microscope, appropriate biohazard containment protocols must be in place and approved by the UMKC Biosafety committee.
Radiation
The confocal microscopy core is not approved to handle radioactive materials and cannot accept any radioactive samples.
Analysis Software
- ImageJ
- Fiji
- Leica offline image processing software
- CellProfiler
- CellProfiler Analyst
- Imaris
- OMERO
- KNIME
How to Use
For best results when affixing a coverslip to a glass slide, use a Toluene-free nail polish such as Wild Shine to seal cover slips on slides.
Check fixation method for your fluorophores and samples. Some fluorophores and tissues require special fixation considerations.
SpectraViewers for common fluorophores
SpectraViewer for common fluorescent proteins
Contact Us
Sarah L. Dallas
Director
David Moore
Co-director