By utilizing cooling Peltier stage and high water vapor pressure in
the specimen chamber (so called "Wet" mode) it is possible to achieve high levels of humidity
(up to 100%). In these conditions wet or hydrated specimens (cells, plant
samples, tissue, etc.) will not dry and introduce any artifacts. Dynamic
experiments are also possible; for example, drying or crystallization processes
can be examined.
Specimen observation at raised chamber pressure without cooling stage (so called “variable pressure” mode)is useful for observing non-conductive specimens without coating. Paper, plastics, ceramics,
fibers, fabrics, outgassing materials – everything goes!
Unlike instruments from other manufacturers, Philips environmental microscopes
provide true secondary electron imaging, and so achieve very high resolution
and can use low beam intensity. This is very important for beam-sensitive
specimens such as biological samples, plastics, textile fibers.
High Vacuum Mode
The main advantage of the XL30 in high vacuum mode is its field-emission
electron gun and advanced electron optics. So, a high resolution/magnification
is obtainable at about any high voltage setting.
High vacuum mode can be divided in two sub modes:
- Low voltage mode – for observing non coated samples. Very low voltage (0.2-1.0
kV) can be used for observation of beam sensitive samples.
- Regular mode – for observing conductive and coated samples. Since useful
resolution is very specimen dependable, this mode usually yields best results
at highest magnifications.